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pet22b vector backbone  (GenScript corporation)

 
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    GenScript corporation pet22b vector backbone
    Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in <t>pET22b</t> vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.
    Pet22b Vector Backbone, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet22b vector backbone/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    pet22b vector backbone - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Repurposing of FDA Approved Drugs Against SARS-CoV-2 Papain-Like Protease: Computational, Biochemical, and in vitro Studies"

    Article Title: Repurposing of FDA Approved Drugs Against SARS-CoV-2 Papain-Like Protease: Computational, Biochemical, and in vitro Studies

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2022.877813

    Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in pET22b vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.
    Figure Legend Snippet: Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in pET22b vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.

    Techniques Used: Plasmid Preparation, Expressing, Synthesized, Purification



    Similar Products

    pet22b vector backbone  (GenScript corporation)
    90
    GenScript corporation pet22b vector backbone
    Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in <t>pET22b</t> vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.
    Pet22b Vector Backbone, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet22b vector backbone/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    pet22b vector backbone - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in pET22b vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.

    Journal: Frontiers in Microbiology

    Article Title: Repurposing of FDA Approved Drugs Against SARS-CoV-2 Papain-Like Protease: Computational, Biochemical, and in vitro Studies

    doi: 10.3389/fmicb.2022.877813

    Figure Lengend Snippet: Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in pET22b vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.

    Article Snippet: The cDNA sequence coding for SCoV-2 PLpro was codon-optimised for bacterial expression and was custom-synthesised in pET22b vector backbone by GeneScript, USA.

    Techniques: Plasmid Preparation, Expressing, Synthesized, Purification